THE ASSESSMENT ON RNA QUALITY AND BANANA RIPENING GENES (MaGAPDH, MaACS1, AND MaACO1) FROM FLESH TISSUES WITH DIFFERENT PRESERVATION METHODS

The preservation of banana fruit is vital to ensure ripening quality during the transportation process. can be defined using gene expression marker, which needs high-quality RNA. However, when access laboratory difficult, long-distance transportation, lack liquid nitrogen or refrigerator, we need an alternative for methods, so sample and extracted RNA are still in excellent conditions. Aim our research find best methods test genes marker Cavendish flesh tissues without nitrogen. In this study, performed extraction by directly storing at -80°C refrigerator combined with use various cutting time (0, 19, 30 minutes). We measured total yield, absorbance ratio 260/280, checked MaGAPDH, MaACS1, MaACO1 Reverse Transcriptase- PCR (RT-PCR). As a result, found that did not affect as well. Although analysis confirmed future quantitative (qPCR) analysis. used method.